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lD-GRAM
SLIDES
INTENDED
USE
ID-GRAM
slide is a multi-purpose microscope slide recommended to
be part of a quality control program to monitor stains and
techniques.
PRINCIPLES
The
slide contains one block (+) with a gram positive control
(Staphylococcus aureus GL0070). The slide also contains
one block (-) with a gram negative control (Escherichia
coli GL0075). The slide contains 6 blank blocks for
the convenience of the technologist to be utilized in performing
clinical Gram stains.
MATERIALS
PRECAUTIONS:
This product is for IN VITRO DIAGNOSTIC USE only. This product
may contain potentially viable organisms. Handle it as you
would a known pathogen.
STORAGE:
Do not freeze or expose to excessive heat. Store at room
temperature and do not use beyond expiration date.
PROCEDURE
(1)
MATERIALS
REQUIRED BUT NOT PROVIDED: The standard clinical microbiological
equipment such as loop, burner, stains are needed for procedures
involving the use of this product.
1. Prepare
smear with a loopful of specimen to be examined within confines
of the blank blocks. In preparing a smear, avoid
harsh
treatment of specimen in order to avoid destruction of
arrangement of cells.
2.
Air dry and heat fix by gentle heating over a bunsen bumer
flame. Do not overheat to avoid possible distortions.
3. Perform
GRAM stain:
a.
FIood entire stain with Gram Crystal Violet for 1
minute.
b.
Wash gently in running water, rinsing both sides.
c.
Add Gram Iodine for 2 minutes.
d.
Wash gently in running water, rinsing both sides.
e.
Decolorize with Gram Decolorizer until solvent flows
colorless from slide.
f.
Counterstain with Gram Safranin for 1 minute.
g.
Wash gently in running water rinsing both sides.
h.
Allow slide to air dry.
i.
Examine under microscope using oil immersion lens.
EXPECTED
RESULTS
Positive
control: purple-blue cocci in clusters (Gram positive)
Negative
control: red rods (Gram negative)
Note:
It is possible that positive control organisms can carry
over to the patient testing area. As a precaution, if a
positive patient result Is recorded, repeat the testing
process on a clean slide that does not contain a positive
control organism.
REFERENCES
1.
Chapin, K. 1995. Clinical Microscopy. Pages 33-51 In P.R.
Murray, E.J. Baron, M.A. Pfaller, F.C. Tenover, and R.H.
Yolken, eds. Manual of Clinical Microbiology. 27:1390-1391.
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